NEK9 polyclonal antibody
产品名称: NEK9 polyclonal antibody
英文名称: NEK9 polyclonal antibody
产品编号: PAB3289
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of NEK9.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NEK9.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Nek9, a novel FACT-associated protein, modulates interphase progression.
Tan BC, Lee SC.J Biol Chem. 2004 Mar 5;279(10):9321-30. Epub 2003 Dec 2.
- 2.
- A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J.J Biol Chem. 2003 Sep 12;278(37):34897-909. Epub 2003 Jul 2.
- 3.
- Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.
Roig J, Mikhailov A, Belham C, Avruch J.Genes Dev. 2002 Jul 1;16(13):1640-58.
- Applications
- Western Blot
- The NEK9 polyclonal antibody (Cat # PAB3289) is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2) .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK9 polyclonal antibody (Cat # PAB3289) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 91754
- Protein Accession#:
- Q8TD19
- Gene Name:
- NEK9
- Gene Alias:
- DKFZp434D0935,MGC138306,MGC16714,NERCC,NERCC1,Nek8
- Gene Description:
- NIMA (never in mitosis gene a)- related kinase 9
- Omim ID:
- 609798
- Gene Ontology:
- Hyperlink
- Other Designations:
- NIMA related kinase 9,NIMA-related kinase Nek8
- Related Disease